<![CDATA[RNA-Seq数据分析]]>http://an.forum.genostack.com/category/33RSS for NodeSat, 13 Jun 2026 14:43:52 GMTThu, 05 Sep 2024 09:10:42 GMT60<![CDATA[siRNA和建模]]>siRNA建模与预测.pptx

]]>http://an.forum.genostack.com/topic/1091/sirna和建模http://an.forum.genostack.com/topic/1091/sirna和建模Thu, 05 Sep 2024 09:10:42 GMT<![CDATA[火山图 小工具]]>和下面的例子一样 需要对RNA的结果展示火山图：
https://training.galaxyproject.org/training-material/topics/transcriptomics/tutorials/rna-seq-viz-with-volcanoplot/tutorial.html#import-data

faee97f7-fec0-4ef7-a486-756d2dceff85-image.png
1.展示火山图
2.标注差异大的基因
3.支持图片版和动态交互版（主要用于对比效果）

一些参考实现：
https://github.com/JoachimGoedhart/VolcaNoseR
https://www.reneshbedre.com/blog/volcano.html
https://thecodingbiologist.com/posts/Making-volcano-plots-in-python-in-Google-Colab
https://hemtools.readthedocs.io/en/latest/content/Bioinformatics_Core_Competencies/Volcanoplot.html

https://medium.com/omics-diary/building-volcano-plots-with-plotly-for-quantitative-analysis-of-omics-data-74e36f4cb8f8

https://bioconductor.org/packages/devel/bioc/vignettes/EnhancedVolcano/inst/doc/EnhancedVolcano.html

js 包
https://github.com/RhoInc/volcano-plot

R
https://github.com/kevinblighe/EnhancedVolcano

]]>http://an.forum.genostack.com/topic/802/火山图-小工具http://an.forum.genostack.com/topic/802/火山图-小工具Tue, 14 Feb 2023 02:55:40 GMT<![CDATA[nanopore dRNAseq]]>https://academic.oup.com/nar/article/50/4/e19/6439677?login=false
Accurate expression quantification from nanopore direct RNA sequencing with NanoCount

]]>http://an.forum.genostack.com/topic/593/nanopore-drnaseqhttp://an.forum.genostack.com/topic/593/nanopore-drnaseqThu, 17 Mar 2022 10:02:59 GMT<![CDATA[RNA-Seq基础知识]]>https://en.wikipedia.org/wiki/Protein_isoform
蛋白异构体
3b07556e-a44e-4028-9fcb-e5f5806797b4-image.png
不同的外显子组合 会编码不同的蛋白质

]]>http://an.forum.genostack.com/topic/546/rna-seq基础知识http://an.forum.genostack.com/topic/546/rna-seq基础知识Sat, 12 Feb 2022 11:55:48 GMT<![CDATA[A content management platform for quantitative omics data]]>https://github.com/Candihub/pixel

]]>http://an.forum.genostack.com/topic/508/a-content-management-platform-for-quantitative-omics-datahttp://an.forum.genostack.com/topic/508/a-content-management-platform-for-quantitative-omics-dataFri, 31 Dec 2021 16:05:22 GMT<![CDATA[GEO 分析指南]]>tutorial.nb.html

https://github.com/sheffield-bioinformatics-core/geo_tutorial/blob/master/tutorial.nb.html

]]>http://an.forum.genostack.com/topic/505/geo-分析指南http://an.forum.genostack.com/topic/505/geo-分析指南Fri, 31 Dec 2021 06:29:58 GMT<![CDATA[hisat2-stringtie 流程]]>http://ccb.jhu.edu/software/stringtie/index.shtml?t=manual
https://github.com/gpertea/stringtie
https://davetang.org/muse/2017/10/25/getting-started-hisat-stringtie-ballgown/

]]>http://an.forum.genostack.com/topic/501/hisat2-stringtie-流程http://an.forum.genostack.com/topic/501/hisat2-stringtie-流程Wed, 22 Dec 2021 07:16:59 GMT<![CDATA[chipster]]>https://chipster.rahtiapp.fi/manual
e24478b1-ab67-4d23-b4d9-e4a25fa92eac-image.png

https://github.com/chipster

]]>http://an.forum.genostack.com/topic/500/chipsterhttp://an.forum.genostack.com/topic/500/chipsterMon, 20 Dec 2021 12:17:23 GMT<![CDATA[FPKM TPM]]>https://www.reneshbedre.com/blog/expression_units.html

]]>http://an.forum.genostack.com/topic/499/fpkm-tpmhttp://an.forum.genostack.com/topic/499/fpkm-tpmMon, 20 Dec 2021 09:19:12 GMT<![CDATA[GO Kegg分析]]>http://combio-sifbi.org/GeneCloudOmics/
https://github.com/buithuytien/GeneCloudOmics
看看这个工具如何和我们集成

]]>http://an.forum.genostack.com/topic/497/go-kegg分析http://an.forum.genostack.com/topic/497/go-kegg分析Fri, 17 Dec 2021 03:21:25 GMT<![CDATA[伯豪RNA-seq流程]]>BC190281-1 真核RNA测序数据分析报告.pdf
主体是一个hisat2 stringtie流程

]]>http://an.forum.genostack.com/topic/496/伯豪rna-seq流程http://an.forum.genostack.com/topic/496/伯豪rna-seq流程Fri, 17 Dec 2021 01:58:45 GMT<![CDATA[RNA-seq分析流程]]>ftp ftp.ccb.jhu.edu

cd pub/RNAseq_protocol
这个目录下有官方论文的示例数据
https://sci-hub.st/10.1038/nprot.2016.095

]]>http://an.forum.genostack.com/topic/476/rna-seq分析流程http://an.forum.genostack.com/topic/476/rna-seq分析流程Fri, 03 Dec 2021 02:07:59 GMT