<![CDATA[三代测序数据分析]]>一，三代测序设备
二，三代测序数据分析
nextdenovo（nextpolish）
last
lastz
cactus
https://nanoporetech.github.io/pomoxis/
三，测序流程
3.1 nanopype
https://nanopype.readthedocs.io/en/latest/
nanopype_workflow.png
7b047f36-6ace-4c40-b76b-da46c0e4fdf9-image.png

basecalling　碱基发现
Albacore　已过时
Flappie 正在试验
Guppy 当前比较推荐的basecalling方法
Guppy-protocol-GPB_2003_v1_revS_14Dec2018-any.pdf
Guppy GPU server mode
Nanopore GUPPY 3.1.5 basecalling on UBUNTU 18.04 using nvidia docker, RTX 2080 & server mode to share GPU machine as a service _ by Winston Koh _ Medium.pdf

3.2 https://timkahlke.github.io/LongRead_tutorials
WF nanopore.png

参考：
１.https://academic.oup.com/bioinformatics/article-abstract/35/22/4770/5514474?redirectedFrom=fulltext
论文：
btz461_supplementary_data.pdf

]]>http://an.forum.genostack.com/topic/7/三代测序数据分析RSS for NodeSat, 13 Jun 2026 13:46:25 GMTMon, 20 Jul 2020 07:43:17 GMT60<![CDATA[Reply to 三代测序数据分析 on Wed, 23 Dec 2020 12:04:17 GMT]]>alignment (Minimap2), variant calling with various tools (Clair, Longshot and Sniffles), and methylation calling (Nanopolish).

]]>http://an.forum.genostack.com/post/259http://an.forum.genostack.com/post/259Wed, 23 Dec 2020 12:04:17 GMT<![CDATA[Reply to 三代测序数据分析 on Wed, 19 Aug 2020 08:14:28 GMT]]>https://github.com/rrwick/Porechop#known-adapters

]]>http://an.forum.genostack.com/post/101http://an.forum.genostack.com/post/101Wed, 19 Aug 2020 08:14:28 GMT<![CDATA[Reply to 三代测序数据分析 on Tue, 11 Aug 2020 07:01:02 GMT]]>btz461_supplementary_data.pdf

]]>http://an.forum.genostack.com/post/85http://an.forum.genostack.com/post/85Tue, 11 Aug 2020 07:01:02 GMT<![CDATA[Reply to 三代测序数据分析 on Tue, 11 Aug 2020 03:20:13 GMT]]>基因学院的培训：
截止日期：2020年8月31日
IP地址：512.tongyuangene.com
用户名：用户姓名全拼
密码：Pass2020 区分大小写，登陆后可以修改密码）。
windows系统：https://pan.baidu.com/s/1ecu1jxx0DxQH4GIvcRiGvQ 提取码：5566
macos系统： https://pan.baidu.com/s/1iKTqsJIM_-9shuiFnbO2oQ 提取码：5566
更多内容，查看附件文档。
Linux预习课程：https://ke.qq.com/course/481801?taid=4387055690275337&tuin=1c7ef510
请从shell与脚本一节开始看即可。课前准备 -mac电脑.pdf 课前准备-windows电脑.pdf 2-纳米孔宏基因组（初稿） .pdf

]]>http://an.forum.genostack.com/post/84http://an.forum.genostack.com/post/84Tue, 11 Aug 2020 03:20:13 GMT<![CDATA[Reply to 三代测序数据分析 on Tue, 04 Aug 2020 12:14:14 GMT]]>naopack
https://github.com/wdecoster/nanopack

]]>http://an.forum.genostack.com/post/73http://an.forum.genostack.com/post/73Tue, 04 Aug 2020 12:14:14 GMT<![CDATA[Reply to 三代测序数据分析 on Fri, 31 Jul 2020 10:13:01 GMT]]>https://zhuanlan.zhihu.com/p/102392867 基因学院　王通

]]>http://an.forum.genostack.com/post/64http://an.forum.genostack.com/post/64Fri, 31 Jul 2020 10:13:01 GMT