<?xml version="1.0" encoding="UTF-8"?><rss xmlns:dc="http://purl.org/dc/elements/1.1/" xmlns:content="http://purl.org/rss/1.0/modules/content/" xmlns:atom="http://www.w3.org/2005/Atom" version="2.0"><channel><title><![CDATA[intermine和bluegene技术验证]]></title><description><![CDATA[<p dir="auto">1.i架构<br />
<img src="/assets/uploads/files/1676862935803-43ad1dc2-8f71-4439-ab7d-b3d9eeaa6fc5-image.png" alt="43ad1dc2-8f71-4439-ab7d-b3d9eeaa6fc5-image.png" class=" img-responsive img-markdown" /><br />
2.安装<br />
<a href="http://intermine.org/im-docs/docs/get-started/tutorial/index" rel="nofollow ugc">http://intermine.org/im-docs/docs/get-started/tutorial/index</a><br />
<img src="/assets/uploads/files/1676863023520-06401b82-117c-470f-af06-0fbb74acbda1-image.png" alt="06401b82-117c-470f-af06-0fbb74acbda1-image.png" class=" img-responsive img-markdown" /></p>
]]></description><link>http://an.forum.genostack.com/topic/812/intermine和bluegene技术验证</link><generator>RSS for Node</generator><lastBuildDate>Sat, 13 Jun 2026 12:33:52 GMT</lastBuildDate><atom:link href="http://an.forum.genostack.com/topic/812.rss" rel="self" type="application/rss+xml"/><pubDate>Mon, 20 Feb 2023 07:42:59 GMT</pubDate><ttl>60</ttl><item><title><![CDATA[Reply to intermine和bluegene技术验证 on Tue, 25 Apr 2023 12:17:42 GMT]]></title><description><![CDATA[<p dir="auto">docker run -p 5000:5000 --env-file /data_raid1/bioinfo/app/bluegene/bluegenes.env -v /data_raid1/bioinfo/app/bluegene/tools:/tools -d --restart unless-stopped intermine/bluegenes:latest</p>
<p dir="auto">docker run -d -v /data_raid1/bioinfo/app/jbrowse/jbrowse_conf.json:/conda/opt/jbrowse/jbrowse_conf.json -v /data_raid1/bioinfo/app/jbrowse/index.html:/conda/opt/jbrowse/index.html -p 8079:80 bokebiotech/jbrowser:v1.16.11</p>
]]></description><link>http://an.forum.genostack.com/post/2071</link><guid isPermaLink="true">http://an.forum.genostack.com/post/2071</guid><dc:creator><![CDATA[zhanglu]]></dc:creator><pubDate>Tue, 25 Apr 2023 12:17:42 GMT</pubDate></item><item><title><![CDATA[Reply to intermine和bluegene技术验证 on Wed, 19 Apr 2023 12:09:55 GMT]]></title><description><![CDATA[<p dir="auto">docker run   -p 8080:8080 -d -v /data_raid1/bioinfo/app/apache-tomcat-10.1.7/webapps/:/usr/local/tomcat/webapps/ tomcat:9.0</p>
<p dir="auto">docker run -p 5000:5000 --env-file /data_raid1/bioinfo/app/bluegene/bluegenes.env -v /data_raid1/bioinfo/app/bluegene/tools:/tools -d --restart unless-stopped intermine/bluegenes:latest</p>
<p dir="auto">docker run -d -v /data_raid1/bioinfo/app/jbrowse/jbrowse_conf.json:/conda/opt/jbrowse/jbrowse_conf.json -v /data_raid1/bioinfo/app/jbrowse/index.html:/conda/opt/jbrowse/index.html -p 8079:80 bokebiotech/jbrowser:v1.16.11</p>
]]></description><link>http://an.forum.genostack.com/post/2045</link><guid isPermaLink="true">http://an.forum.genostack.com/post/2045</guid><dc:creator><![CDATA[zhanglu]]></dc:creator><pubDate>Wed, 19 Apr 2023 12:09:55 GMT</pubDate></item><item><title><![CDATA[Reply to intermine和bluegene技术验证 on Wed, 19 Apr 2023 10:38:35 GMT]]></title><description><![CDATA[<p dir="auto">gradle.properties添加<br />
org.gradle.jvmargs=-Xmx10096m -XX:MaxPermSize=10096m -XX:+HeapDumpOnOutOfMemoryError -Dfile.encoding=UTF-8</p>
]]></description><link>http://an.forum.genostack.com/post/2044</link><guid isPermaLink="true">http://an.forum.genostack.com/post/2044</guid><dc:creator><![CDATA[zhanglu]]></dc:creator><pubDate>Wed, 19 Apr 2023 10:38:35 GMT</pubDate></item><item><title><![CDATA[Reply to intermine和bluegene技术验证 on Wed, 12 Apr 2023 07:17:47 GMT]]></title><description><![CDATA[<h2>Intermine+bluegene+jbrow部署配置</h2>
<ol>
<li>intermine<br />
（1）编译</li>
</ol>
<pre><code>git clone https://github.com/intermine/biotestmine
cd biotestmine
./gradlew war
</code></pre>
<p dir="auto">注意：执行编译打包时会因为网络问题，下载依赖失败，配上aliyun或者腾旭仓库后会下载依赖会提速，但intermine的jar包无法下载；</p>
<p dir="auto">（2）导入数据：<br />
启动数据库</p>
<pre><code>docker run -d -p 8983:8983 -v /data_raid1/bioinfo/app/solr-7.2.1/data:/var/solr/data -t --privileged=true solr:8.11.1

docker run  -v /data_raid1/bioinfo/app/postgresql_data:/var/lib/postgresql/data -e POSTGRES_PASSWORD=postgres -p 5432:5432 -d postgres:10
</code></pre>
<p dir="auto">修改数据库配置文件</p>
<pre><code>mkdir ~/.intermine
cp ./biotestmine/data/biotestmine.properties ~/.intermine/
</code></pre>
<p dir="auto">使用 样例数据：biotestmine/data/malaria-data.tar.gz</p>
<pre><code>tar -zxvf malaria-data.tar.gz
cp biotestmine/data/project.xml biotestmine
</code></pre>
<p dir="auto">配置project数据路径<br />
导入数据：</p>
<pre><code>./gradlew buildDB
./gradlew integrate -Psource=uniprot-malaria --stacktrace
./gradlew integrate -Psource=malaria-gff --stacktrace
./gradlew integrate -Psource=malaria-chromosome-fasta --stacktrace
./gradlew integrate -Psource=go --stacktrace
./gradlew integrate -Psource=go-annotation --stacktrace
./gradlew integrate -Psource=update-publications --stacktrace
./gradlew integrate -Psource=entrez-organism --stacktrace

./gradlew postprocess -Pprocess=create-references
./gradlew postprocess -Pprocess=transfer-sequences
./gradlew postprocess -Pprocess=summarise-objectstore

solr create -c biotestmine-search
solr create -c biotestmine-autocomplete

./gradlew postprocess -Pprocess=create-search-index
./gradlew postprocess -Pprocess=create-autocomplete-index
</code></pre>
<p dir="auto">（3）启动biotestmine</p>
<pre><code>docker run   -p 8080:8080 -d -v /data_raid1/bioinfo/app/apache-tomcat-10.1.7/webapps/:/usr/local/tomcat/webapps/ tomcat:9.0
</code></pre>
<p dir="auto">将war包映射到tomcat下，使用docker方式启动；<br />
配置文件介绍<br />
（1）配置postgre数据库：webapps/biotestmine/WEB-INF/classes/intermine.properties<br />
（2）配置solr数据库,在webapps/biotestmine/WEB-INF/classes/ 新建keyword_search.properties</p>
<pre><code>index.solrurl = http://192.114.101.5:8983/solr/biotestmine-search
index.batch.size = 1000
autocomplete.solrurl = http://192.114.101.5:8983/solr/biotestmine-autocomplete
</code></pre>
<p dir="auto">（3）biotestmine/WEB-INF/web.properties</p>
<pre><code>webapp.baseurl=http://103.114.101.5:8080
webapp.deploy.url=http://103.114.101.5:8080
</code></pre>
<p dir="auto">注意：<br />
webapps/biotestmine/WEB-INF/classes/intermine.properties下有webapp.baseurl和webapp.deploy.url的配置，但是web.properties优先级更高；</p>
]]></description><link>http://an.forum.genostack.com/post/2029</link><guid isPermaLink="true">http://an.forum.genostack.com/post/2029</guid><dc:creator><![CDATA[zhanglu]]></dc:creator><pubDate>Wed, 12 Apr 2023 07:17:47 GMT</pubDate></item><item><title><![CDATA[Reply to intermine和bluegene技术验证 on Fri, 31 Mar 2023 07:51:02 GMT]]></title><description><![CDATA[<p dir="auto">我们使用intermine来构建这三个薯类物种<br />
<a href="https://data.jgi.doe.gov/refine-download/phytozome" rel="nofollow ugc">https://data.jgi.doe.gov/refine-download/phytozome</a><br />
manihot esculenta v8.1 木薯    taxid 3983<br />
<a href="https://www.ncbi.nlm.nih.gov/search/all/?term=manihot+esculenta" rel="nofollow ugc">https://www.ncbi.nlm.nih.gov/search/all/?term=manihot+esculenta</a><br />
Solanum tuberosum v6.1 马铃薯<br />
<a href="https://www.ncbi.nlm.nih.gov/search/all/?term=Solanum+tuberosum" rel="nofollow ugc">https://www.ncbi.nlm.nih.gov/search/all/?term=Solanum+tuberosum</a>  taxid 4113</p>
<p dir="auto">甘薯: taxid:4120<br />
<a href="https://www.ncbi.nlm.nih.gov/search/all/?term=Ipomoea%20batatas" rel="nofollow ugc">https://www.ncbi.nlm.nih.gov/search/all/?term=Ipomoea batatas</a><br />
（<a href="http://public-genomes-ngs.molgen.mpg.de/sweetpotato/DOWNLOADS/%EF%BC%89" rel="nofollow ugc">http://public-genomes-ngs.molgen.mpg.de/sweetpotato/DOWNLOADS/）</a><br />
lpomoea batatas<br />
ipoBat4.fa   基因组<br />
ipoBat4.transcript.bed12  基因<br />
ipoBat4.transcript.fa  转录本<br />
ipoBat4_final.mod.vcf.gz  变异</p>
]]></description><link>http://an.forum.genostack.com/post/1952</link><guid isPermaLink="true">http://an.forum.genostack.com/post/1952</guid><dc:creator><![CDATA[anneng]]></dc:creator><pubDate>Fri, 31 Mar 2023 07:51:02 GMT</pubDate></item><item><title><![CDATA[Reply to intermine和bluegene技术验证 on Wed, 22 Feb 2023 02:42:37 GMT]]></title><description><![CDATA[<p dir="auto">biotestmine验证<br />
biotestmine是intermine的一个测试项目<br />
<a href="https://github.com/intermine/biotestmine" rel="nofollow ugc">https://github.com/intermine/biotestmine</a></p>
<p dir="auto">验证过程：<br />
clone项目<br />
git clone <a href="https://ghproxy.com/https://github.com/intermine/biotestmine.git" rel="nofollow ugc">https://ghproxy.com/https://github.com/intermine/biotestmine.git</a><br />
1.安装perl依赖</p>
<pre><code>sudo cpan XML::Parser::PerlSAX Text::Glob Cwd Getopt::Std
</code></pre>
<p dir="auto">cpan网络不稳定 perl的包都可以直接用ubuntu的apt安装</p>
<p dir="auto">2.安装postgresql<br />
最好不要用root  使用ubuntu当前用户在pg中创建一个role</p>
<pre><code>CREATE USER role_name(和ubuntu的一个本地用户同名);
alter role role_name with CREATEDB;
</code></pre>
<p dir="auto">参考<br />
<a href="https://www.digitalocean.com/community/tutorials/how-to-use-roles-and-manage-grant-permissions-in-postgresql-on-a-vps-2" rel="nofollow ugc">https://www.digitalocean.com/community/tutorials/how-to-use-roles-and-manage-grant-permissions-in-postgresql-on-a-vps-2</a><br />
3.安装Solr</p>
<pre><code>//下载时间比较长 找我获取
wget https://archive.apache.org/dist/lucene/solr/8.9.0/solr-8.9.0.tgz
bash solr-8.9.0/bin/install_solr_service.sh solr-8.9.0.tgz
sudo ufw allow 8983
./bin/solr create_core -c demo
./bin/post -c demo2 example/exampledocs/*xml
</code></pre>
<p dir="auto"><a href="http://192.168.0.157:8983/solr/#/demo2/core-overview" rel="nofollow ugc">http://192.168.0.157:8983/solr/#/demo2/core-overview</a><br />
biotestmine怎么使用Solr的还需要细看下<br />
<a href="https://intermine.readthedocs.io/en/latest/system-requirements/software/solr/" rel="nofollow ugc">https://intermine.readthedocs.io/en/latest/system-requirements/software/solr/</a><br />
4.创建配置目录<br />
mkdir ~/.intermine<br />
cp biotestmine/data/biotestmine.properties ~/.intermine<br />
修改PSQL_USER and PSQL_PWD为PG的新建role_name(实际上就是ubuntu的一个本地用户)</p>
<p dir="auto">5.执行安装<br />
DB_ENCODING=UTF-8 ./setup.sh<br />
由于maven比较慢 使用腾讯镜像<br />
vi build.gradle</p>
<pre><code>buildscript {
    repositories {
**maven { url 'https://mirrors.cloud.tencent.com/nexus/repository/maven-public/' }**
        mavenCentral()
    }

    dependencies {
        classpath group: 'org.intermine', name: 'plugin', version: System.getProperty("imVersion")
        classpath 'com.bmuschko:gradle-cargo-plugin:2.8.0'
    }
}

ext {
    mineRelease = hasProperty('release') ? "." + release : ''
    mineName = "biotestmine"
    minePropertyFileName = "${mineName}.properties$mineRelease"
    minePropertyFile = "${System.env.HOME}/.intermine/$minePropertyFileName"
    if( !file(minePropertyFile).exists() ) {
        minePropertyFile = "$project.rootDir/dbmodel/resources/biotestmine.properties"
    }
    println "Using properties file: '$minePropertyFile'"
}

subprojects {
    apply plugin: 'java'
    apply plugin: 'maven'

    sourceCompatibility = 1.8
    targetCompatibility = 1.8

    repositories {
**maven { url 'https://mirrors.cloud.tencent.com/nexus/repository/maven-public/' }**
        mavenLocal()
        mavenCentral()
        maven {
            url "https://clojars.org/repo"
        }
    }

</code></pre>
<p dir="auto"><img src="/assets/uploads/files/1677032285587-bc9630df-3d64-4a82-8e34-1751a47fe5cc-image.png" alt="bc9630df-3d64-4a82-8e34-1751a47fe5cc-image.png" class=" img-responsive img-markdown" /></p>
<p dir="auto">其他参考<br />
<a href="http://intermine.org/im-docs/docs/get-started/tutorial/index" rel="nofollow ugc">http://intermine.org/im-docs/docs/get-started/tutorial/index</a><br />
<a href="https://github.com/intermine/biotestmine" rel="nofollow ugc">https://github.com/intermine/biotestmine</a></p>
]]></description><link>http://an.forum.genostack.com/post/1951</link><guid isPermaLink="true">http://an.forum.genostack.com/post/1951</guid><dc:creator><![CDATA[anneng]]></dc:creator><pubDate>Wed, 22 Feb 2023 02:42:37 GMT</pubDate></item></channel></rss>