@zhanglu /opt/app/genostack_v3_service/genostack_tool/amplicon_analysis_16s.yaml

sda 8:0 0 10.7T 0 disk
sdb 8:16 0 10.7T 0 disk
sdc 8:32 0 10.7T 0 disk
sdd 8:48 0 10.7T 0 disk
sde 8:64 0 10.7T 0 disk
sdf 8:80 0 10.7T 0 disk
sdg 8:96 0 10.7T 0 disk
sdh 8:112 0 10.7T 0 disk
sdi 8:128 0 10.7T 0 disk
sdj 8:144 0 10.7T 0 disk
sdk 8:160 0 10.7T 0 disk
sdl 8:176 0 10.7T 0 disk
sdm 8:192 0 10.7T 0 disk
sdn 8:208 0 10.7T 0 disk
sdo 8:224 0 10.7T 0 disk
sdp 8:240 0 10.7T 0 disk
sdq 65:0 0 10.7T 0 disk
sdr 65:16 0 10.7T 0 disk
sds 65:32 0 10.7T 0 disk
sdt 65:48 0 10.7T 0 disk
└─sdt1 65:49 0 10.7T 0 part /public5
sdu 65:64 0 10.7T 0 disk
└─sdu1 65:65 0 10.7T 0 part /public4
sdv 65:80 0 10.7T 0 disk
└─sdv1 65:81 0 10.7T 0 part /public3
sdw 65:96 0 10.7T 0 disk
└─sdw1 65:97 0 10.7T 0 part /public2
sdx 65:112 0 10.7T 0 disk /public1
sdy 65:128 0 10.7T 0 disk
├─sdy1 65:129 0 512M 0 part /boot/efi
├─sdy2 65:130 0 500M 0 part /boot
├─sdy3 65:131 0 32G 0 part
└─sdy4 65:132 0 10.7T 0 part /

@zhanglu dockerhub.genostack.com:8090/library/mongo:latest

63343368-47a1-427b-a972-d398f02c244e-image.png

mountvolume.setup failed for volume "kubesphere-config" : configmap "kubesphere-config" not found

查询报错信息，显示没有kubesphere-config这个configmap。应该去创建kubesphere-config这个configmap。但是在kubesphere的文档上看到有人调整了ks-apiserver的limit memory就好了，我查看ks-apiserver默认的limit memorr设置的是1G，我调整成4G,没想到ks-apiserver服务还真运行起来了。我也很纳闷，configmap跟内存有啥关系，先不管了。继续处理问题。

测试

gpu time /cephfs_data/app/blast_gpu/ncbi-blast-2.2.28+-src/c++/GCC480-ReleaseMT64/bin/blastp -query /cephfs_data/app/blast_gpu/database/test2.fa -db /cephfs_data/app/blast_gpu/database/nr -gpu t -num_threads 30 real 13m42.160s user 38m52.082s sys 0m13.170s cpu

time /cephfs_data/app/blast_gpu/ncbi-blast-2.2.28+-src/c++/GCC480-ReleaseMT64/bin/blastp -query /cephfs_data/app/blast_gpu/database/test2.fa -db /cephfs_data/app/blast_gpu/database/nr -gpu f -num_threads 30

real 23m23.076s
user 695m50.953s
sys 1m35.377s

启动x11vnc 最后一个链接断开时，不停止vnc

-forever

x11vnc -forever

quantms

定量质谱工作流程。目前支持具有 DDA-LFQ、DDA-Isobaric 和 DIA-LFQ 定量复杂实验设计的蛋白质组学实验。

nextflow run quantms/main.nf \ --outdir result \ --labelling_type "label free sample" \ --input '/ceph_disk3/mengpf/work/Project/Nextflow/quantms/data/BSA_design_urls.tsv' \ --database '/ceph_disk3/mengpf/work/Project/Nextflow/quantms/data/18Protein_SoCe_Tr_detergents_trace_target_decoy.fasta' \ --posterior_probabilities "fit_distributions" \ --search_engines "msgf,comet" \ --decoy_string "rev" \ --add_triqler_output true \ --protein_level_fdr_cutoff 1.0 \ --psm_level_fdr_cutoff 1.0 \ --acquisition_method "dda" \ --quantify_decoys true \ -profile docker

/opt/app/elasticsearch/data.json 备份到node3:/public3/node1/elasticsearch

配置文件的优先级
Selector priority

When mixing generic process configuration and selectors the following priority rules are applied (from lower to higher):

Process generic configuration.

Process specific directive defined in the workflow script.

withLabel selector definition.

withName selector definition.

sudo systemctl start rsync.service
sudo systemctl status rsync.service
sudo systemctl enable rsync.service

数据库备份：
gk_l.sql
gk_g.sql

https://github.com/sqjin/CellChat

根据设置外网不能访问配置方式：

#修改配置 vi docker/.env SESSION_COOKIE_SAMESITE=None SESSION_COOKIE_SECURE=False SESSION_COOKIE_HTTPONLY=False WTF_CSRF_ENABLED=False DATABASE_PORT= DATABASE_DIALECT= POSTGRES_DB= POSTGRES_USER= POSTGRES_PASSWORD= REDIS_HOST= REDIS_PORT= #将ports修改为:0.0.0.0:port vi docker-compose-non-dev.yml superset: env_file: docker/.env-non-dev image: *superset-image container_name: superset_app command: ["/app/docker/docker-bootstrap.sh", "app-gunicorn"] user: "root" restart: unless-stopped ports: - 0.0.0.0:6013:8088 #修改版本 TAG=2.1.0 docker-compose -f superset/docker-compose-non-dev.yml up -d