ORF 开放阅读框

anneng

open reading frames (ORFs) are defined as spans of DNA sequence between the start and stop codons.
三个碱基确定一个氨基酸因此一个序列会产生不同的解读可能例如下图 ATG（启动子）不同的阅读顺序会导致其出现在不同的位置

Six-frame translation
DNA有双链一个链产生三种一共6个阅读框 blastx 会用着6个阅读框去蛋白库进行搜索找出来匹配的蛋白从而确定序列具体的阅读框位置

anneng

https://ib.bioninja.com.au/options/untitled/b2-biotechnology-in-agricul/gene-identification.html

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https://sci-hub.ru/10.1016/j.tig.2017.12.009
The Definition of Open Reading Frame Revisited

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https://linsalrob.github.io/ComputationalGenomicsManual/ORFCalling/