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    Directional/stranded RNA-seq data

    生物信息分析
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    • A
      anneng 最后由 编辑

      https://chipster.csc.fi/manual/library-type-summary.html9c6f772f-0e3f-4b95-9864-a73ff9838a34-image.png
      The reads on the left are from the same strand as the transcript, and their pairs on the right are from the opposing strand. The number above the read states which read it is, the first (/1) or the second (/2). Thus, perhaps a bit unintuitively, the first case, "fr-firststrand" is such that the first read (/1) is actually from the opposing strand as the transcript, and second read (/2) is from the transcript strand.

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      • A
        anneng 最后由 编辑

        https://www.ecseq.com/support/ngs/how-do-strand-specific-sequencing-protocols-work

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