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blast在查询时会查两个方向

生物信息分析

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anneng 最后由编辑

在这篇文献中提到了3对引物每对的第二个引物都是另外一个方向对齐的例如 5′- GATCTTTGTGGTCATTCTCTTC-3′ 这个引物
https://www.nature.com/articles/s41598-018-34147-7
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