12小鼠nanopore数据分析
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docker run -v /ceph_disk2/data/hongyuan/mnt/data/find_data/mouse_12_dongyangchao/mouse_throat_swab/20220521_1551_MN28730_FAR09113_370180dd/fast5/:/workhome genomicpariscentre/guppy-gpu:6.0.1 guppy_basecaller -i /workhome/raw -s /workhome/fastq --barcode_kits "EXP-NBD104" -c dna_r9.4.1_450bps_hac.cfg --num_callers 2 --cpu_threads_per_caller 1 --gpu_runners_per_device 20
docker的拆分有问题 直接使用命令行命令
sudo guppy_basecaller --do_read_splitting --detect_mid_strand_adapter --detect_mid_strand_barcodes -i fast5 -s fastq --barcode_kits "EXP-NBD104" -c dna_r9.4.1_450bps_hac.cfg --num_callers 2 --cpu_threads_per_caller 1 --gpu_runners_per_device 20 -x 'auto'