art pbsim3 模拟数据

[zhanglu]

二代： art

https://www.niehs.nih.gov/research/resources/software/biostatistics/art/index.cfm

Dockerfile

from ubuntu:20.04
run apt update && apt-get install -y wget && wget https://www.niehs.nih.gov/research/resources/assets/docs/artbinmountrainier2016.06.05linux64.tgz
run tar -zxvf artbinmountrainier2016.06.05linux64.tgz
run mv art_bin_MountRainier/art_illumina /usr/bin/ && rm artbinmountrainier2016.06.05linux64.tgz

运行命令：

art_illumina -sam -i seq_reference.fa -l 50 -f 10 -o ./outdir/dat_single_end
art_illumina -p -sam -i seq_reference.fa -l 50 -f 20 -m 200 -s 10 -o ./outdir/dat_paired_end