细胞群内成对分组间差异表达分析

anneng

/home/bioinfo/ms_jk_small_tool/分组间差异分析+报告展示/代码/细胞群内成对分组间差异表达分析

conda install -c conda-forge r-base
conda install anndata -c conda-forge
conda install -c conda-forge r-curl
conda install -c conda-forge r-ragg
conda install -c anaconda click
conda install -c bioconda scanpy
conda install -c plotly plotly
conda install -c conda-forge python-kaleido
conda install -c bioconda bioconductor-clusterprofiler
pip install dfply

R
install.packages("Rcpp")
install.packages('remotes')
install.packages('pkgdown')
install.packages('devtools')
install.packages('R.utils')
install.packages('sqldf')
install.packages('ggpubr')
install.packages('ggupset')
if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")
BiocManager::install("clusterProfiler")

library('devtools')
install_local('seurat-wrappers')  这个包比较特殊 下从git下载到本地  再安装
install_local('presto')

anneng

File "/home/bioinfo/workspace/miniconda3/envs/snakemake2/lib/python3.11/site-packages/click/core.py", line 754, in invoke
return __callback(*args, **kwargs)
^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/home/bioinfo/workspace/miniconda3/envs/snakemake2/lib/python3.11/site-packages/click/decorators.py", line 26, in new_func
return f(get_current_context(), *args, **kwargs)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/home/bioinfo/ms_jk_small_tool/分组间差异分析+报告展示/代码/细胞群内成对分组间差异表达分析/workflow/scripts/split_violin_plot.py", line 110, in split_violin_plot
marker_gene = top_up.append(top_down)
^^^^^^^^^^^^^
File "/home/bioinfo/workspace/miniconda3/envs/snakemake2/lib/python3.11/site-packages/pandas/core/generic.py", line 5989, in getattr
return object.getattribute(self, name)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
AttributeError: 'DataFrame' object has no attribute 'append'. Did you mean: '_append'?
https://stackoverflow.com/questions/75956209/error-dataframe-object-has-no-attribute-append