@mengpf https://github.com/shangshanzhizhe/Work_flow_of_population_genetics/blob/master/Work_flows/structure_variation.md
三种软件的使用

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9434330/
Research progress on application of single-cell TCR/BCR sequencing technology to the tumor immune microenvironment, autoimmune diseases, and infectious diseases
单细胞的几个应用场景：
Single-cell sequencing technologies mainly include
single-cell transcriptome sequencing,
single-cell assay for transposase accessible chromatin with high-throughput sequencing,
single-cell immune profiling (single-cell T-cell receptor [TCR]/B-cell receptor [BCR] sequencing), and single-cell transcriptomics.
93a86f70-df5d-4805-8d55-6d1ba7368a24-image.png

sudo systemctl start rsync.service
sudo systemctl status rsync.service
sudo systemctl enable rsync.service

kubeadm 证书时间100年

清理无用镜像
docker image prune -a

大服务器gitlab

docker run
-itd
-p 9880:9880
-p 9822:22
-v /data_raid1/gitlab/etc:/etc/gitlab
-v /data_raid1/gitlab/log:/var/log/gitlab
-v /data_raid1/gitlab/opt:/var/opt/gitlab
--privileged=true
twang2218/gitlab-ce-zh:latest

root/Qiankun2021

https://tech.olx.com/running-spark-on-kubernetes-a-fully-functional-example-and-why-it-makes-sense-for-olx-d56b6a61fcbe

数据库备份：
gk_l.sql
gk_g.sql

https://github.com/sqjin/CellChat

File "/home/bioinfo/workspace/miniconda3/envs/snakemake2/lib/python3.11/site-packages/click/core.py", line 754, in invoke
return __callback(*args, **kwargs)
^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/home/bioinfo/workspace/miniconda3/envs/snakemake2/lib/python3.11/site-packages/click/decorators.py", line 26, in new_func
return f(get_current_context(), *args, **kwargs)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File "/home/bioinfo/ms_jk_small_tool/分组间差异分析+报告展示/代码/细胞群内成对分组间差异表达分析/workflow/scripts/split_violin_plot.py", line 110, in split_violin_plot
marker_gene = top_up.append(top_down)
^^^^^^^^^^^^^
File "/home/bioinfo/workspace/miniconda3/envs/snakemake2/lib/python3.11/site-packages/pandas/core/generic.py", line 5989, in getattr
return object.getattribute(self, name)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^^
AttributeError: 'DataFrame' object has no attribute 'append'. Did you mean: '_append'?
https://stackoverflow.com/questions/75956209/error-dataframe-object-has-no-attribute-append