节点142重启后需要关闭防火墙
systemctl stop firewalld

setenforce 0
sed -i '/SELINUX/s/enforcing/disabled/' /etc/selinux/config
systemctl stop firewalld && systemctl disable firewalld
iptables -F && iptables -X && iptables -F -t nat && iptables -X -t nat && iptables -P FORWARD ACCEPT
swapoff -a && free –h
service dnsmasq stop && systemctl disable dnsmasq

启动x11vnc 最后一个链接断开时，不停止vnc

-forever

x11vnc -forever

https://file.genostack.com/public_resource/local-path-storage.yaml

accfbcb2-ee0c-4d81-a2f3-c767256839c3-image.png

https://bitbucket.org/rirgabiss/mhinngs/src/master/
MHinNGS is a tool for analysis of microhaplotypes (MHs) in singleend sequencing data obtained through a massive parallel sequencing plattform (MPS). The tool identifies the reads with the MHs and calls the genotypes of the MHs according to the criteria and positions specified in the configuration file. It also searches for additional variants in the region defined by the start and the stop positions specified in the configuration file.
这个软件的输入是参考序列和原始单端测序的fastq 包括了比对过程 依赖的第三方软件包括
python 3.6 including pip
samtools version 1.9
hisat2 version 2.1.0
stringtie version 2.0.3
agrep T.R.E. version 0.8.0
不支持双端序列

quantms

定量质谱工作流程。目前支持具有 DDA-LFQ、DDA-Isobaric 和 DIA-LFQ 定量复杂实验设计的蛋白质组学实验。

nextflow run quantms/main.nf \ --outdir result \ --labelling_type "label free sample" \ --input '/ceph_disk3/mengpf/work/Project/Nextflow/quantms/data/BSA_design_urls.tsv' \ --database '/ceph_disk3/mengpf/work/Project/Nextflow/quantms/data/18Protein_SoCe_Tr_detergents_trace_target_decoy.fasta' \ --posterior_probabilities "fit_distributions" \ --search_engines "msgf,comet" \ --decoy_string "rev" \ --add_triqler_output true \ --protein_level_fdr_cutoff 1.0 \ --psm_level_fdr_cutoff 1.0 \ --acquisition_method "dda" \ --quantify_decoys true \ -profile docker

参考

https://gatk.broadinstitute.org/hc/en-us/articles/360035531572-Evaluating-the-quality-of-a-germline-short-variant-callset

问题：Pacbio HIFI 16s流程跑qiime diversity core-metrics-phylogenetic会报错

解决： qiime diversity core-metrics-phylogenetic中有一个内容会自动检测GPU，然后调用GPU，如果检测到GPU，但是调用失败则会报错。

在跑命令之前先运行 export UNIFRAC_USE_GPU=N即可使用CPU而不用GPU