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conda create aligners
1.序列比对 mafft --auto /mnt/hdfs/biodata/FMDV/sequences.fasta > fmdv_all.aln 2.裁剪 trimal -in fmdv_all.aln -out fmdv.aln.trimmed 3.建树 fasttree -gamma -nt -gtr -out fmdv.tree fmdv.aln.trimmed 整体下来1小时30分钟 10000多条序列