单细胞数据分析概要
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单细胞类型注释:
https://www.cellkb.com/
https://www.biostars.org/p/433235/
scCATCH(https://github.com/ZJUFanLab/scCATCH)
SCSA (https://github.com/bioinfo-ibms-pumc/SCSA).
https://www.frontiersin.org/articles/10.3389/fgene.2020.00490/full
SCSA: A Cell Type Annotation Tool for Single-Cell RNA-seq Data
单细胞待解决的问题:
https://openproblems.bio/ -
scapy 培训视频
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https://scanpy.discourse.group/t/highly-variable-genes-best-practice/29/5
scanpy hvgs
You can find our current best-practices in the recent publication here 98. Typically the pre-processing steps in an analysis workflow would be:Cell & Gene QC
Normalization
Batch correction (or data integration)
HVG selection
Dimensionality reduction (including visualization) -
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https://www.singlecellcourse.org/
sanger的单细胞教程 -
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https://squidpy.readthedocs.io/en/latest/
squidpy 空间转录组分析 -