https://www.sc-best-practices.org/air_repertoire/ir_profiling.html
56a55e24-8f0e-4b43-a73b-0ee7beaf8d4e-image.png

VDJ过程
adc29db5-511a-48a1-bd0f-e9269ac0a751-image.png

Definition of Gene Therapy in the EU
In the EU, the definition of a Gene Therapy Medicinal Product (GTMP) is provided
in Directive 2009/120/EC amending Directive 2001/83/EC, part IV of Annex I.
A GTMP means a biological medicinal product that has the following
characteristics:
(a) it contains an active substance that contains or consists of a recombinant
nucleic acid used in or administered to human beings with a view to
regulating, repairing, replacing, adding or deleting a genetic sequence;
(b) its therapeutic, prophylactic or diagnostic effect relates directly to the
recombinant nucleic acid sequence it contains, or to the product of the
genetic expression of this sequence.
GTMPs do not include vaccines against infectious diseases.
Hazel Aranha, Humberto Vega-Mercado - Handbook of Cell and Gene Therapy_ From Proof-of-Concept through Manufacturing to Commercialization-CRC Press (2023).pdf

核磁在代谢组学中的应用

1.核磁共振技术原理
https://www.youtube.com/watch?v=pUWcXvw1Rsg
https://www.bilibili.com/video/BV1CU4y1E7xL/?spm_id_from=333.788.recommend_more_video.-1(有中文翻译 比较好)

https://bitbucket.org/rirgabiss/mhinngs/src/master/
MHinNGS is a tool for analysis of microhaplotypes (MHs) in singleend sequencing data obtained through a massive parallel sequencing plattform (MPS). The tool identifies the reads with the MHs and calls the genotypes of the MHs according to the criteria and positions specified in the configuration file. It also searches for additional variants in the region defined by the start and the stop positions specified in the configuration file.
这个软件的输入是参考序列和原始单端测序的fastq 包括了比对过程 依赖的第三方软件包括
python 3.6 including pip
samtools version 1.9
hisat2 version 2.1.0
stringtie version 2.0.3
agrep T.R.E. version 0.8.0
不支持双端序列

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828144/
Practical Guidelines for the Comprehensive Analysis of ChIP-seq Data
3d1f0fc6-d2ed-4539-9cde-0c03bde51949-image.png

https://github.com/mesoscope

参考

https://gatk.broadinstitute.org/hc/en-us/articles/360035531572-Evaluating-the-quality-of-a-germline-short-variant-callset

https://www.sinobiological.com/resource/protein-review/fc-fusion-proteins
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1689181129856.gif

https://civicdb.org/pages/about