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    • 免疫组学

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      A

      https://www.sc-best-practices.org/air_repertoire/ir_profiling.html
      56a55e24-8f0e-4b43-a73b-0ee7beaf8d4e-image.png

      VDJ过程
      adc29db5-511a-48a1-bd0f-e9269ac0a751-image.png

    • 细胞与基因疗法 cell and gene therapy

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      A

      Definition of Gene Therapy in the EU
      In the EU, the definition of a Gene Therapy Medicinal Product (GTMP) is provided
      in Directive 2009/120/EC amending Directive 2001/83/EC, part IV of Annex I.
      A GTMP means a biological medicinal product that has the following
      characteristics:
      (a) it contains an active substance that contains or consists of a recombinant
      nucleic acid used in or administered to human beings with a view to
      regulating, repairing, replacing, adding or deleting a genetic sequence;
      (b) its therapeutic, prophylactic or diagnostic effect relates directly to the
      recombinant nucleic acid sequence it contains, or to the product of the
      genetic expression of this sequence.
      GTMPs do not include vaccines against infectious diseases.
      Hazel Aranha, Humberto Vega-Mercado - Handbook of Cell and Gene Therapy_ From Proof-of-Concept through Manufacturing to Commercialization-CRC Press (2023).pdf

    • 代谢组学

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      A

      核磁在代谢组学中的应用

      1.核磁共振技术原理
      https://www.youtube.com/watch?v=pUWcXvw1Rsg
      https://www.bilibili.com/video/BV1CU4y1E7xL/?spm_id_from=333.788.recommend_more_video.-1(有中文翻译 比较好)

    • 司法

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      A

      https://bitbucket.org/rirgabiss/mhinngs/src/master/
      MHinNGS is a tool for analysis of microhaplotypes (MHs) in singleend sequencing data obtained through a massive parallel sequencing plattform (MPS). The tool identifies the reads with the MHs and calls the genotypes of the MHs according to the criteria and positions specified in the configuration file. It also searches for additional variants in the region defined by the start and the stop positions specified in the configuration file.
      这个软件的输入是参考序列和原始单端测序的fastq 包括了比对过程 依赖的第三方软件包括
      python 3.6 including pip
      samtools version 1.9
      hisat2 version 2.1.0
      stringtie version 2.0.3
      agrep T.R.E. version 0.8.0
      不支持双端序列

    • 表观遗传学

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      A

      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828144/
      Practical Guidelines for the Comprehensive Analysis of ChIP-seq Data
      3d1f0fc6-d2ed-4539-9cde-0c03bde51949-image.png

    • 合成生物学

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      A

      https://github.com/mesoscope

    • 植物基因组学

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      M

      参考

      https://gatk.broadinstitute.org/hc/en-us/articles/360035531572-Evaluating-the-quality-of-a-germline-short-variant-callset

    • 蛋白组学

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      A

      https://www.sinobiological.com/resource/protein-review/fc-fusion-proteins
      46c1ca94-e2a9-44bd-b53e-593a82afcd3c-image.png

    • 临床生物信息

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      A

      1689181129856.gif

    • 肿瘤NGS数据分析

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      A

      https://civicdb.org/pages/about

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      突变分析
      • anneng

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    • A

      lncRna 分析
      • anneng

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      A

      https://www.lncrnablog.com/

    • A

      Directional/stranded RNA-seq data
      • anneng

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      https://www.ecseq.com/support/ngs/how-do-strand-specific-sequencing-protocols-work

    • A

      Transcript Summary
      • anneng

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    • A

      注释数据库
      • anneng

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      A

      https://www.well.ox.ac.uk/ogc/guide-reference-genome-selection/
      A guide to reference genome selection
      95ecab75-e638-45e6-bf32-3e0d8f313acd-image.png

    • A

      Is there any difference between HG 19 (from the UCSC) and GRCh37(NCBI)?
      • anneng

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      There are a few minor differences between GRCh37 and hg19. The contig sequences are the same but the names are different, i.e. "1" may need to be converted to "chr1". In addition UCSC hg19 is currenly using the old mitochondrial sequence but NCBI and Ensembl have transitioned to NC_012920.
      Citing UCSC:
      "Since the release of the UCSC hg19 assembly, the Homo sapiens mitochondrion sequence (represented as "chrM" in the Genome Browser) has been replaced in GenBank with the record NC_012920. We have not replaced the original sequence, NC_001807, in the hg19 Genome. We plan to use the Revised Cambridge Reference Sequence (rCRS) in the next human assembly release."

    • A

      功能注释
      • anneng

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    • A

      微生态的多元分析 Multivariate analyses in microbial ecology
      • anneng

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      A

      https://fukamilab.github.io/BIO202/index.html
      Biology 202: Ecological Statistics
      Stanford University

    • A

      Introduction to the Analysis of Environmental Sequences: Metagenomics with MEGAN
      • anneng

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    • A

      此主题已被删除!
      • anneng

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    • A

      bam 报没有RG头
      • anneng

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    • A

      QuickNGS
      • anneng

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    • A

      使用sqlite来保存fastq文件
      • anneng

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    • A

      宏基因质控
      • anneng

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    • A

      宏基因组装软件分析
      • anneng

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      A

      https://edwards.sdsu.edu/crass/#
      跨样本组装

      https://my.oschina.net/u/4599872/blog/4472869

    • A

      Computational Genomics Tutorial
      • anneng

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      https://github.com/linsalrob/ComputationalGenomicsManual

    • A

      生物软件的容器化方案
      • anneng

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    • A

      如何将R变成API?
      • anneng

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    • A

      Homologue, orthologue and Paralogue-Bioinformatics
      • anneng

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    • A

      从centrifuge的结果中提取某个物种的序列
      • anneng

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