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    • 免疫组学

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      https://www.sc-best-practices.org/air_repertoire/ir_profiling.html
      56a55e24-8f0e-4b43-a73b-0ee7beaf8d4e-image.png

      VDJ过程
      adc29db5-511a-48a1-bd0f-e9269ac0a751-image.png

    • 细胞与基因疗法 cell and gene therapy

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      Definition of Gene Therapy in the EU
      In the EU, the definition of a Gene Therapy Medicinal Product (GTMP) is provided
      in Directive 2009/120/EC amending Directive 2001/83/EC, part IV of Annex I.
      A GTMP means a biological medicinal product that has the following
      characteristics:
      (a) it contains an active substance that contains or consists of a recombinant
      nucleic acid used in or administered to human beings with a view to
      regulating, repairing, replacing, adding or deleting a genetic sequence;
      (b) its therapeutic, prophylactic or diagnostic effect relates directly to the
      recombinant nucleic acid sequence it contains, or to the product of the
      genetic expression of this sequence.
      GTMPs do not include vaccines against infectious diseases.
      Hazel Aranha, Humberto Vega-Mercado - Handbook of Cell and Gene Therapy_ From Proof-of-Concept through Manufacturing to Commercialization-CRC Press (2023).pdf

    • 代谢组学

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      核磁在代谢组学中的应用

      1.核磁共振技术原理
      https://www.youtube.com/watch?v=pUWcXvw1Rsg
      https://www.bilibili.com/video/BV1CU4y1E7xL/?spm_id_from=333.788.recommend_more_video.-1(有中文翻译 比较好)

    • 司法

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      https://bitbucket.org/rirgabiss/mhinngs/src/master/
      MHinNGS is a tool for analysis of microhaplotypes (MHs) in singleend sequencing data obtained through a massive parallel sequencing plattform (MPS). The tool identifies the reads with the MHs and calls the genotypes of the MHs according to the criteria and positions specified in the configuration file. It also searches for additional variants in the region defined by the start and the stop positions specified in the configuration file.
      这个软件的输入是参考序列和原始单端测序的fastq 包括了比对过程 依赖的第三方软件包括
      python 3.6 including pip
      samtools version 1.9
      hisat2 version 2.1.0
      stringtie version 2.0.3
      agrep T.R.E. version 0.8.0
      不支持双端序列

    • 表观遗传学

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      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828144/
      Practical Guidelines for the Comprehensive Analysis of ChIP-seq Data
      3d1f0fc6-d2ed-4539-9cde-0c03bde51949-image.png

    • 合成生物学

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      https://github.com/mesoscope

    • 植物基因组学

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      M

      参考

      https://gatk.broadinstitute.org/hc/en-us/articles/360035531572-Evaluating-the-quality-of-a-germline-short-variant-callset

    • 蛋白组学

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      https://www.sinobiological.com/resource/protein-review/fc-fusion-proteins
      46c1ca94-e2a9-44bd-b53e-593a82afcd3c-image.png

    • 临床生物信息

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      1689181129856.gif

    • 肿瘤NGS数据分析

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      A

      https://civicdb.org/pages/about

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      中草药相关文献
      • anneng

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      http://cbcb.cdutcm.edu.cn/TCMPG/resource/genomes/
      Although hundreds of medical plants have been sequenced, the related data were scattered at different sources, which hindered the systematical researches on medical plants. To solve such a challenge, we proposed the integrative database for Traditional Chinese Medicine Plant Genome (TCMPG). TCMPG not only deposits the genome data of traditional Chinese medical plants, but also provides a series of computational tools for researchers to perform systematical analysis. At present, 160 kinds of plants, 195 corresponding genomes, and 255 herbs (different parts from the same plant), together with the related information on plant species, genomes, and herbal medicines were included in TCMPG.
      Moreover, the main genomic analysis tools, namely the BLAST to find orthologs from different plant, SSR Finder to identify SSRs, JBrowse to browse genomes, Synteny Viewer to display the syntenic blocks between two genomes, and HmmSearch to identify protein domain, were also embedded in TCMPG, which will facilitate the systematical analysis on medical plants. Once new data was reported, TCMPG will be updated and improved in time.

    • A

      seven bridges 七桥的CWL流程仓库
      • anneng

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      SB metadata.png

      七桥的metadata管理 星号的我们直接继承过来
      七桥的metadata不能在UI上自定义 只能通过上传工具和API自定义 我们要把这一块补齐

    • A

      香农商的应用场景
      • anneng

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      A

      gutjnl-2017-November-66-11-2013-inline-supplementary-material-1.pdf
      4b476875-8198-4367-9c89-900240588283-image.png

    • A

      scikit-bio 项目
      • anneng

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      sequence logos
      • anneng

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      https://github.com/ffrancis/Multiple-sequence-alignment-Shannon-s-entropy

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      有参和无参组装
      • anneng

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    • A

      12小鼠nanopore数据分析
      • anneng

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    • A

      SAM文件解读
      • anneng

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      https://www.biostars.org/p/356811/
      d39c720f-9e7f-43d4-9aae-9d45679b87a8-image.png

    • A

      对于SAM中 clip的解释
      • anneng

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    • A

      docker镜像来源
      • anneng

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    • A

      paleogenomics-tor-vergata 一个古基因教程
      • anneng

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    • A

      环状参考序列的处理
      • anneng

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      https://paleogenomics-tor-vergata.readthedocs.io/en/latest/5_ReadsMapping_v2.html

    • A

      Circdna 环状dna发现流程
      • anneng

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    • A

      nf-core/eager:A fully reproducible and state-of-the-art ancient DNA analysis pipeline
      • anneng

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    • A

      amplication vs shotgun
      • anneng

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    • A

      Amplicon analysis 扩增子分析
      • anneng

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      Ultraplex: A rapid, flexible, all-in-one fastq demultiplexer
      • anneng

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      mutation rate
      • anneng

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      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5100877/
      54204a64-de65-4ed3-a01c-3ae567d09058-image.png
      这篇文章直接使用突变的个数和总序列数进行比较 计算突变频率

    • A

      qrqc 对序列质量进行评估
      • anneng

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    • A

      blastn多线程
      • anneng

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